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Several researchers have demonstrated the utility of such a method to produce chimeric phages with the ability to infect bacteria that were previously resistant to the phage These studies have been accomplished using T-even phages The adsorption speci city of phages in the T4-like virus genus is determined by the protein sequence near the tip of the long tail bers (the distal tail ber locus) (T tart et al 1998) The tail ber adhesin domains are located within different genes in closely related phages of the T-even type For example, in phage T4, the adhesin sequence is encoded by the C-terminal domain of the large tail ber gene (gene 37), while phage T2 encodes the adhesin as a separate gene product (gp) (gene 38) that binds to the tip of the T2 tail bers (34).

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The result of integrating the dyadic QQ of the final relative momentum after the collision over all impact conditions du can depend only on the initial conditions PP and the unit tensor. We can hence further simplify (7.173) to obtain the final result

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Three groups of T-even phages that possess the tail ber organization of phage T2 have been identi ed, including the T2-like, T6-like, and Ac3-like sequences (T tart et al 1998) The sequences of genes 37 and 38 vary to differing degrees among these phages T tart et al (1996, 1998) were the rst to show that T4-like phages have conserved regions anking their host range genes, and that these conserved sequences could be used to amplify the host range genes (genes 37 and 38) of many T4-like phages For the T4-like phages, there are two completely different organizations of the distal tail ber locus the T2 and T4 forms The sequences of gene 37 and gene 38 vary to differing degrees among the T4-like phages, while those of gene 36 and gene t, which ank the locus, are conserved (Yu et al 2000).

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Using the conserved regions of gene 36 and gene t, T tart et al (1996) ampli ed a DNA segment of two Yersinia T4-like phages that contained genes 37 and 38, cloned each fragment into a plasmid, and used a phage T4 mutant that had amber mutations in genes 37 and 38 (am37, am38) to infect cells carrying each of the plasmids Chimeric T4 phages, which replaced their distal tail ber locus with the plasmid-borne analog, were isolated and shown to form plaques on Yersinia (T tart.

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4 7 Rev isit the exact anal ys is of the inverting amp lifie r in Section 4. 3. (a) Find an expression for the vo ltage gain if Rin

$result = 2 * 2 + 3 * 3 + 5; (= 4 + 3 * 3 + 5) //imaginary evaluation steps (= 4 + 9 + 5) (= 13 + 5) (= 18)

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