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The Cache-Control general header gives an elaborate access to the cache and was discussed earlier in this chapter. In brief, its main purpose is to give better control of what is to be cached versus not cached and a scheme on how to calculate the freshness lifetime of a resource to determine whether or not it is stale.

p(X i = xi | parents (X i ))

Clinical psychology is changing and growing at a rapid pace. Some of these changes are very positive; some clearly negative. On the positive side, psychology has greatly contributed to a better understanding of human behavior and ways to improve the quality of life for many. Assessment, treatment, research, teaching, and consultation are all

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38 Preparation of BMP-2 334 39 Preparation of TGF- 1 335 4 Isolation and Culture Methodology of hMSC 335 41 Isolation of Mesenchymal Stem Cells 337 Protocol 134 Isolation of hMSCs from Bone Marrow 337 42 Thawing and Maintenance of hMSC Cell Culture 339 Protocol 135 Expansion of Human Mesenchymal Stem Cells 339 5 Characterization of hMSCS 340 51 Authentication of Surface Antigen Analysis 341 Protocol 136 FACS Analysis of Human Mesenchymal Stem Cells 341 52 Pellet Culture of Bone 342 Protocol 137 Osteogenic Differentiation in Pellet Culture 342 53 Pellet Culture of Cartilage 343 Protocol 138 Chondrogenic Differentiation in Pellet Culture 343 6 Tissue Engineering of Bone 343 61 Preparation of Spinner Flask 344 Protocol 139 Culture of hMSCs on Silk-RGD Scaffolds in Spinner Flasks 344 7 Analytical Assays 347 71 DNA Assay .

The net effect is that when the user changes worksheets or workbooks, the following CheckGridlines procedure is executed. This procedure ensures that the check mark displayed on the GridLines menu option is in sync with the sheet.

348 Protocol 1310 DNA Assay of Cells Cultured on Scaffolds or as Pellets 348 72 ALP Assay 351 Protocol 1311 Assay of Alkaline Phosphatase Activity in Bone Constructs 351 73 Calcium Assay 353 Protocol 1312 Calcium Assay in Bone Constructs 353 74 Glycosaminoglycan (GAG) Assay 355 Protocol 1313 Assay of GAGs in Cartilage and Bone Constructs 355 75 TRIzol RNA Extraction 357 Protocol 1314 RNA Extraction from Bone Constructs 357 76 Real-Time RT-PCR 360 Protocol 1315 Gene Expression Analysis in Bone and Cartilage Constructs 360 8 Histology 363 Protocol 1316 Fixation and Paraf n Embedding of Bone and Cartilage Constructs 363 Protocol 1317 Von Kossa Staining of Sections of Bone Constructs 364 Protocol 1318 Safranin-O Staining of Sections of Cartilage Constructs 365 9 In Vitro Applications 366 Sources of Materials 368 References 370.

CReplObject *pObject = NULL; CFolder *pFolder; CItem *pItem; UINT uType = *(PUINT)lpb; lpb += sizeof(uType); switch(uType) { case OT_FOLDER: pObject = pFolder = new CFolder; pFolder->m_uSyncOpt = *(PUINT)lpb; lpb += sizeof(pFolder->m_uSyncOpt); break; case OT_ITEM: pObject = pItem = new CItem; pItem->m_uid = *(PUINT)lpb; lpb += sizeof(pItem->m_uid); pItem->m_ftModified = *(FILETIME *)lpb; lpb += sizeof(pItem->m_ftModified); break; } return (HREPLOBJ)pObject; } The service manager passes a byte array of length cb. The service provider proceeds by extracting the parts of that array it is interested in as defined by the CItem and CFolder data members. Once it has finished, the provider returns a handle to the new object. As we will see in the next section, the service manager uses these handles during object enumeration.

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Attributes:

PURPOSE NESTED WITHIN START TAG END TAG CONTENT ATTRIBUTES: Demarcates the portion of the document containing HTML None (encloses all other elements) Optional Optional Contains HTML elements and text lang = "language-code" (OPTIONAL) dir = (ltr, rtl) (OPTIONAL)

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onClipEvent (enterFrame) { mydate = new Date(); hours = mydate.getHours(); minutes = mydate.getMinutes(); seconds = mydate.getSeconds(); // Calculate value of AMorPM variable before changing hours variable to compensate for military time if (hours<12) { AMorPM = AM ; } else { AMorPM = PM ; } // At midnight military time =0 if (hours<1) { hours = 12; } if (hours>12) { hours = hours-12; } if (minutes<10) { minutes = 0 +minutes; } if (seconds<10) { seconds = 0 +seconds; } current_time = hours+ : +minutes+ : +seconds+ +AMorPM; }